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5.362 Kinetics of Enzyme Inhibition (New)

Prereq: 5.07[J] or 7.05, 5.352 or 5.310 Coreq: 5.361
U (Spring; partial term)
5 Units

Students study the activity and structure of the domains developed in 5.361 to understand the role of mutations in the development of resistance to Gleevec. Students assay both mutant and wild-type Abl kinase domains for phosphorylation activity to determine enzyme kinetics and the inhibition efficacy of Gleevec. They conduct additional testing on kinase activity of Gleevec-resistant mutants in the presence of other potential inhibitors. Uses structure-viewing programs to enable analysis of the mechanistic basis of Bcr-Abl inhibition and Gleevec-resistance.

B. Pentelute

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Chemical Engineering (Course 10)

...4 Combination of 5.361 Expression and Purification of Enzyme Mutants , 5.362 Kinetics of...